pnt2 cell line  (DS Pharma Biomedical)

Journal: British Journal of Cancer

Article Title: Peroxisomal β-oxidation enzyme, DECR2, regulates lipid metabolism and promotes treatment resistance in advanced prostate cancer

doi: 10.1038/s41416-023-02557-8

Figure Lengend Snippet: a Illustration of fatty acid oxidation in the peroxisome and mitochondria. Thioridazine (TDZ) is an inhibitor of perFAO. b Heatmap of peroxisomal β-oxidation (perFAO) gene expression in Taylor and Grasso cohorts. We manually curated a list of perFAO genes based on Gene Ontology pathway. Cell viability of ( c ) castrate-resistant C42B, 22Rv1 and V16D, and enzalutamide-resistant MR49F prostate cancer cell lines across a range of TDZ doses. d C42B and 22Rv1 prostate cancer cell lines treated with 2.5 µM TDZ were assessed for cell migration using transwell migration assay. Scale bar: left, 100 µm; right, 200 µm. e Immunostaining for proliferative marker Ki67 in vehicle (VEH) or TDZ-treated (20 μM) patient-derived explants (PDEs). Immunohistochemical staining and quantification of the proliferative marker Ki67 is shown ( n = 11). Scale bar, 50 µm. f DECR2 expression with respect to tumour progression in four independent datasets. DECR2 levels were analysed in normal, primary, and metastatic castrate-resistant or neuroendocrine tissue samples. g The association of DECR2 expression and disease-free survival in the MSKCC (Taylor) cohort. h DECR2 protein expression in non-malignant prostate cell lines (PNT1 and PNT2) and prostate cancer cell lines (LNCaP, VCaP, C42B, 22Rv1, V16D, PC3), including enzalutamide-resistant prostate cancer cell line (MR49F). i Left : Representative DECR2 IHC staining of benign prostate tissues and prostate cancer tissues. Scale bar, 50 µm. Middle : DECR2 protein expression in a validation cohort consisting of benign prostate tissues ( n = 3) and prostate cancer tissues ( n = 10). Right : Intra-tissue IHC analysis of DECR2 expression in prostate cancer tissues ( n = 10). All cell line data are representative of at least two independent experiments and presented as mean ± s.e.m of triplicate wells. Statistical analysis was performed using ordinary one-way ANOVA or two-tailed student’s t test. Data in ( e ) were statistically analysed using paired t-test. Data in ( g ) were statistically analysed using a two-sided log-rank test. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

Article Snippet: Human immortalised normal prostate epithelial cell lines PNT1 and PNT2 were obtained from the European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Gene Expression, Migration, Transwell Migration Assay, Immunostaining, Marker, Derivative Assay, Immunohistochemical staining, Staining, Expressing, Immunohistochemistry, Biomarker Discovery, Two Tailed Test

Journal: bioRxiv

Article Title: TGF-β induces matrisome pathological alterations and EMT in patient-derived prostate cancer tumoroids

doi: 10.1101/2023.04.03.534859

Figure Lengend Snippet: (A) Representative two-photon images of PNT2 cells taken after 10 days of colonization of two tumour dECMs obtained from two different patients. The images represent maximum projections of 3D z-stack images. Cells were stained with DAPI. The colour code refers to the depth to which nuclei are found inside the dECM. Scale bars: 100 µm. (B) Representative confocal image of PNT2 cells cultured for 10 days on tumour dECM. The cells are stained for vimentin (VIM, red), F-actin (green) and nuclei are counterstained DAPI (blue). Scale bar: 25 µm. (C) Flow cytometry dot plots of vimentin and E-cadherin expression for PNT2 cells, after a week of incubation with 2D and 3D dECMs, in the presence or absence of TGF-β. CTRL cells grown directly on the surface of culture flasks are also shown. (D) Vimentin expression quantification based on flow cytometry analysis. Statistical analysis was performed by two-way ANOVA. (E) Representative dot plot of PCTs containing E-cadherin (E-CAD), vimentin and dual positive cells. Histogram representation of the epithelial cells from the PCTs expressing vimentin, for control condition and cultured in 3D dECMs in the presence or absence of TGF-β inhibitor (F) Vimentin expression quantification based on flow cytometry analysis, for E-CAD-positive cells. Statistical analysis was performed by one-way ANOVA. (G) Graphical representation of the experimental design and the results obtained.

Article Snippet: PNT2 human cell line was purchased from Sigma Aldrich (95012613-1VL) and cultured in RPMI 1640 containing 2 mM Glutamine and 10 % Foetal Bovine Serum (FBS), as suggested by the manufacturer.

Techniques: Staining, Cell Culture, Flow Cytometry, Expressing, Incubation

Journal: PLoS ONE

Article Title: Antitumor Activity of Hierridin B, a Cyanobacterial Secondary Metabolite Found in both Filamentous and Unicellular Marine Strains

doi: 10.1371/journal.pone.0069562

Figure Lengend Snippet: Cytotoxicity of 1 towards a panel of human cell lines. (+ growth inhibition observed, − no growth inhibition observed).

Article Snippet: Hepatocellular carcinoma cell line HepG2, colon adenocarcinoma cell line HT-29, neuroblastoma cell line SH-SY5Y, breast carcinoma cell line T47D and the normal prostate cell line PNT2 were purchased from Sigma-Aldrich.

Techniques: Inhibition